Hemostasis and Thrombosis Unit

Hemostasia and Thrombosis Unit


This Unit is in charge of the study and management of the coagulation diseases, including thrombotic and hemorrhagic diseases. It also performs clinical activity and represents the Central Laboratory (Figure 1).

The head of the Hemostasia and Thrombosis Unit is Dr. Santiago Bonanad. The Unit is located on the 4th floor in Tower A. The Unit performs research and special studies on hemostasia in close collaboration with the Accredited Group of Hemostasia, Thrombosis, Arterioesclerosis and Vascular Biology led by Dr. Francisco España. Another important collaboration is established with the Genetics Unit, led by Dr. José Cervera, in order to share resources for advanced genetic studies in the area of Hemostasia. The Unit also uses the service of the Central Laboratory to perform urgent or regular non specialized tests. One long-term goal is the integration within the Central Laboratory, so the clinical knowledge can contribute to the analytical activity.


The Hemostasia and Thrombosis Unit is a reference center at the international level for the management of patients with congenital coagulopathies, together with other hospitals such as

Hospital La Paz (Madrid), Hospital Vall d‘Hebron (Barcelona) and Virgen del Rocío (Seville). These are the four national centers of reference for the management of congenital coagulopathies. The Unit has recently applied for the nomination as Reference Center for congenital coagulopathies management within the CSUR Program (Centros, Servicios y Unidades de Referencia del Sistema Nacional de Salud). The center belongs to the network CIEHEMO of International centers with training in hemophilia and related diseases, within the World Foundation in hemophilia. We are the unique center of Spanish language outside the United States offering training programs to professionals dedicated to hemophilia and related diseases.


The activity of the Unit is focused on three areas: external patients, hospitalization and laboratory of Hemostasia and Thrombosis.


Reference laboratory in hemostasia


  1. Laboratory of coagulation and immunology
  • Determination of coagulation factors
  • Autoimmunity (antiphospholipids)
  • Thrombin generation assays
  • Study of ADAMTS-13 metalloprotease (activity and inhibitors)
  • Detection of coagulation inhibitors
  • Characterization of anti FVIII inhibitors
  • Determination of IgG subtypes
  • Screening (Kasper) and quantification (Bethesda)
  • Study of von Willebrand factor (VWF)
  • Visualization of the multimeric structure of VWF
  • Quantification of VWF propeptide
  • Study and isolation of fibrinogen
  • Quantification of fibrinogen by protein techniques
  • Study of kinetics in plasma
  • Study of kinetics in isolated fibrinogen
  • SDS-PAGE gel electrophoresis
  • Generation of thrombin
  • Search of mutations causing the phenotype by molecular biology techniques


  1. Laboratory of genetic diagnosis in coagulopathies
  • Hemophilia A
  • Detection of F8 intron 22 inversion
  • Detection of F8 intron 1 inversion
  • Sequencing of Factor VIII (F8) gene
  • Study of carriers: analysis of the presence of mutations responsible for familiar coagulopathies
  • Search of F8 deletions and duplications through multiplex ligation-dependent probe amplification (MLPA) analysis
  • Prenatal diagnosis
  • Hemophilia B
  • Sequencing of Factor IX gene
  • Search of FIX deletions and duplications through multiplex ligation-dependent probe
  • von Willebrand disease
  • Sequencing of VWF gene
  • Sequencing of discrete domains of VWF gene (exon 28, domains D´-D3, CK)
  • Search of VWF deletions and duplications through multiplex ligation-dependent probe amplification (MLPA) analysis